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  • Neogen® Petrifilm® Bacillus cereus Count Plates

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    • Neogen® Petrifilm® Bacillus cereus Count Plates

    Neogen® Petrifilm® Bacillus cereus Count Plates

    Neogen® Petrifilm® Bacillus cereus Count Plates provide a rapid and standardized method for enumerating Bacillus cereus in food and environmental samples.
    Selective media allow typical blue colonies to form in about 24 hours.
    Ideal for rice, pasta, spices, dairy, meat products and hygiene monitoring in food facilities.
    Model : 6483
    • LF-6483-仙人掌桿菌-中文DM.pdf
    • LF-仙人掌桿菌-使用說明.pdf

    • Description
    • Specifications
    • Features
    • Applications

    Neogen® Petrifilm® Bacillus cereus Count Plates (BC Plates) are designed specifically for the enumeration of Bacillus cereus in a wide range of foods and environmental samples.
    To perform the test, inoculate 1 mL of diluted sample onto the plate and spread evenly using the Petrifilm spreader.

    The plates contain selective components, growth inhibitors and indicator dyes that allow Bacillus cereus to develop as clearly identifiable blue colonies.
    The transparent background and printed grid support fast, consistent and accurate counting.

    BC Plates are suitable for rice, pasta, spices, dairy products, meat products, processed foods and food-contact surfaces.
    Most results can be read within 24 hours, making the method ideal for routine QC and batch release testing.

    BrandPetrifilm®
    Product Name Neogen® Petrifilm® Bacillus cereus Count Plates (BC)
    Target Organism Bacillus cereus
    Sample Types Rice, pasta, spices, dairy, meat, processed foods, food-contact surfaces
    Incubation Conditions 24 ± 2 hours at 30–35°C
    Reading Typical colonies appear blue; transparent background with printed grid
    Recommended Count Range 15–150 CFU per plate (higher levels require additional dilution)
    Catalog Numbers 25 plates/box
    Storage Condition Refrigerated or frozen at 2–8°C
    Brand Neogen® Petrifilm®
    Method Validation AOAC International / AFNOR (depending on food category)

Advantages

  • No agar preparation or pour plating required, saving significant time.
  • Typical blue colonies make results clear and easy to interpret.
  • Selective media suppress background organisms for more accurate counts.
  • Thin-film format saves incubator space and increases throughput.
  • Suitable for a wide variety of foods and environmental samples.

Limitations

  • Not intended for clinical diagnostic use; for food and environmental testing only.
  • Acidic or antimicrobial foods require verification for method suitability.
  • High bacterial loads may cause TNTC results and require further dilution.
  • Some Bacillus species may produce atypical colonies; confirmation may be required.

Operating Notes

  • When inoculating 1 mL, hold the pipette vertically and avoid bubbles while spreading.
  • Blue colony morphology should be confirmed based on size and appearance.

Technical Details

  • Growth area approximately 20 cm² with printed grid for accurate enumeration.
  • Most samples yield readable results within 24 hours.
  • Contains selective agents to inhibit non-target flora.
  • Aligned with AOAC validated principles for Bacillus cereus enumeration.
  • Bacillus cereus enumeration in starch-based foods such as rice and pasta.
  • Microbiological quality control of spices, powders and prepared foods.
  • Routine testing of dairy, meat and processed foods.
  • Hygiene monitoring of equipment, surfaces and tools in food facilities.
  • Used by third-party laboratories and training centers for food microbiology education.

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